FGFR4 Antibody

• Catalog Number: orb215947
• Category: Antibodies
• Description: FGFR4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human FGFR4 recombinant protein (Position: L22-H206). Human FGFR4 shares 86% and 84% amino acid (aa) sequences identity with mouse and rat FGFR4, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 87954 MW
• UniProt ID: P22455
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Fibroblast growth factor receptor 4;FGFR-4;2.7.10.
• Note: For research use only
• Application notes: WB: The detection limit for FGFR4 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of FGFR4 using anti-FGFR4 antibody.Lane 1:recombinant human FGFR4 protein 0.5 ng.

WB analysis of FGFR4 using anti-FGFR4 antibody.Lane 1:human HeLa cell; 2:human PANC cell; 3:human SGC cell; 4:human COLO320 cell; 5:human SW620 cell.

IHC analysis of FGFR4 using anti-FGFR4 antibody. FGFR4 was detected in a paraffin-embedded section of human lung cancer tissue.