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Catalog Number | orb1263053 |
---|---|
Category | Antibodies |
Description | FGFR2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-P, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 794-821 amino acids from the C-terminal region of human FGFR2. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 92 kDa |
Target | FGFR2 |
UniProt ID | P21802 |
NCBI | P21802 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Fibroblast growth factor receptor 2, FGFR-2, K-sam Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-hFGFR2-C808 Pab in 293 cell line lysates transiently transfected with the FGFR2 gene (2ug/lane)
Western blot analysis of anti-FGFR2 Pab in NCI-H460 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of FGFR2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
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