FGFR1 (phospho-Y654) antibody

• Catalog Number: orb213927
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FGFR1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y654 of human FGFR1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FGFR1
• Entrez: 79114, 14182, 2260
• UniProt ID: Q04589, P16092, P11362
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti BFGFR antibody, anti CEK antibody, anti FGFBR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FGFR1 (Phospho-Y654) expression in mouse testis (A) whole cell lysates. (Predicted band size: 91 kD; Observed band size: 120 kD)

Immunofluorescent analysis of FGFR1 (Phospho-Y654) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.