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Catalog Number | orb1272622 |
---|---|
Category | Antibodies |
Description | FGF17 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, NeA, WB |
Reactivity | Human |
Immunogen | E.coli derived Recombinant Human FGF-17 |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | FGF17 |
UniProt ID | O60258 |
NCBI | O60258 |
Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | HH20, FGF-13Fibroblast growth factor 17FGF-17 Read more... |
Note | For research use only |
Application notes | ELISA:Sandwich:To detect FGF-17 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with BIorbyt’s Polyclonal Biotinylated Anti-Human FGF-17 (38-295) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Western Blot:: To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant FGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of FGF-17 (200.0 ng/mL), a concentration of 2.1 – 3.2 μg/mL of this antibody is required. |
Expiration Date | 12 months from date of receipt. |
To detect hFGF-17 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-17 (orb1272614) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17.
To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
IH, WB | |
Human, Mouse, Primate, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
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