FFAR1 antibody

• Catalog Number: orb318893
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPR40
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR40. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FFAR1
• Entrez: 233081, 2864, 266607
• UniProt ID: O14842, Q76JU9, Q8K3T4
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti GPR40 antibody, anti Free fatty acid receptor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPR40 expression in SGC7901 (A), A2780 (B), Panc1 (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 31 kD)

Immunofluorescent analysis of GPR40 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.