Ferritin antibody

• Catalog Number: orb20090
• Category: Antibodies
• Description: Goat polyclonal antibody to Ferritin
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 2-4 μg/ml
• Conjugation: Unconjugated
• MW: 21.2
• Target: FTH1
• Entrez: 2495
• Protein Sequence: DKHTLGDSDNES
• RRID: AB_10755883
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-Cell proliferation-inducing gene 15 protein a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 22kDa band observed in Human Brain (Cerebellum), Liver and Placenta lysates (calculated MW of 21.2kDa according to NP_002023.2). Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Placenta lysate using Ferritin antibody

Immunohistochemical staining of Human Liver using Ferritin antibody

orb20090 (2 µg/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb20090 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

orb20090 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.