FEN1 Antibody

• Catalog Number: orb1672068
• Category: Antibodies
• Description: FEN1 Antibody
• Clonality: Recombinant
• Clone Number: SAIC-21C-4
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: The KLH-cojugated peptide "SIEEIVR", derived from the Flap endonuclease 1 involved in DNA replication and repair.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: FEN1
• UniProt ID: P39748
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Flap endonuclease, RAD2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of HeLa cells with anti-FEN1 (SAIC-21C-4). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-21C-4 (orb1672068) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672068- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-FEN1 antibody SAIC-21C-4. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-21C-4 (orb1672068 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot using anti-FEN1 antibody SAIC-21C-4. Human colon tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-21C-4 (orb1672068) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.