Fcgr2b Antibody

• Catalog Number: orb1672260
• Category: Antibodies
• Description: Fcgr2b Antibody
• Clonality: Recombinant
• Clone Number: 2.4G2
• Tested applications: Blocking, FC, IF, IHC, IP
• Reactivity: Mouse
• Isotype: IgG2b kappa
• Immunogen: Mouse BALB/c Macrophage J774 Cell Line.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Fcgr2b
• UniProt ID: P08101, P08508
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Fcγ III/II Receptor, Low affinity immunoglobulin g
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate - left and middle panel) as well as bone marrow-derived macrophages (BMDMs - right panel) were stained with a murine anti-human antibody (fluorescently lAF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (orb1672260 - clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently lntibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.

Anti-CD16/32 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/32 antibody orb1672260 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially avail a competitor. Compared to the isotype control and secondary-only control- a CD16/32-positive population is apparent.