FCER2 Antibody

• Catalog Number: orb1671641
• Category: Antibodies
• Description: FCER2 Antibody
• Clonality: Recombinant
• Clone Number: CAT-13.9C1
• Tested applications: IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising mice with with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: FCER2
• UniProt ID: P06734
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Low affinity IgE Receptor, Low affinity immunoglob
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD23 antibody CAT-13.9C1. Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of CAT-13.9C1 (orb1671641 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed Daudi cells with anti-CD23 antibody CAT-13.9C1. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version CAT-13.9C1 (orb1671641) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671641- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.