Fc gamma receptor III Antibody [3G8], Rabbit IgG

• Catalog Number: orb1671874
• Category: Antibodies
• Description: Fc gamma receptor III Antibody [3G8], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 3G8
• Tested applications: Blocking, Depletion, FC, IHC, IP
• Reactivity: Human, Marmoset, Monkey, Primate
• Isotype: IgG kappa
• Immunogen: 3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: Q9ULV2
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD16, low affinity IgG receptor III, FcγRIII, low
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-Fc gamma receptor III antibody 3G8. Human peripheral blood leukocytes were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control - black line) or the r-chimeric version of 3G8 (orb1671874 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed U937 cells with anti-Fc gamma receptor III antibody 3G8. Immunofluorescence analysis of paraformaldehyde fixed U937 cells permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of 3G8 (orb1671874) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671874- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor® 488 secondary antibody.