FBXL5 Antibody (N-term)

• Catalog Number: orb1927933
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23907
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:100, FC: 1:10~50, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 78555 Da
• Target: This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the N-terminal region of human FBXL5.
• UniProt ID: Q9UKA1
• NCBI: NP_001180464.1, NP_036293.1, NP_001180463.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: F-box/LRR-repeat protein 5, F-box and leucine-rich
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FBXL5 Antibody (N-term) in mouse heart tissue lysates (35 ug/lane). FBXL5 (arrow) was detected using the purified Pab.

FBXL5 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-FBXL5 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lane 3: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 79 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.