Fbp5A antibody

• Catalog Number: orb345454
• Category: Antibodies
• Description: Fbp5A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human Fbp5A protein.
• Concentration: 1.3 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 1:500 - 1:3,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against human Fbp5A protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Fbp5A protein from human and chimpanzee based on 100% homology with the immunizing sequence. Expect partial reactivity with Fbp5A from dog based on partial (~92%, 13/14) sequence homology. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q9UKT4
• NCBI: 6912366
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-F-Protein Only Protein 5/Fbp5A antibod
• Note: For research use only
• Application notes: This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 51 kDa in size corresponding to Fbp5A protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Biorbyt's Affinity Purified anti-Fbp5a antibody shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Western blot using Biorbyt's affinity purified anti-Fbp5A antibody shows detection of a major band corresponding to Fbp5A protein. Lane 1: HeLa whole cell lysate (p/n orb348668). The identity of cross-reactive minor bands at ~72 kDa and 150 kDa is unknown. Lane 2: Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide. Approximately 33 µg of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:500 dilution in BLOTTO (p/n orb348624). The membrane was washed and reacted with a 1:10000 dilution of IRDye™800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.