Fascin Antibody

• Catalog Number: orb749791
• Category: Antibodies
• Description: Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM133
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the Fascin antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Fascin antibody is available for research use only.
• UniProt ID: Q16658
• Storage: Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with Fascin antibody (clone SPM133).

Immunofluorescent staining of permeabilized human HeLa cells with Fascin antibody (clone SPM133, green), Phalloidin (actin, red) and Hoescht (nucleus, blue).

Flow cytometry testing of PFA-fixed human K562 cells with Fascin antibody (clone SPM133); Red=isotype control, Blue=Fascin antibody.

Western blot testing of Fascin antibody and human HeLa cell lysate (clone SPM133). Predicted molecular weight ~55 kDa.

SDS-PAGE analysis of purified, BSA-free Fascin antibody (clone SPM133) as confirmation of integrity and purity.