FANCA antibody

• Catalog Number: orb345459
• Category: Antibodies
• Description: FANCA antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 995-1009 of human FANCA protein.
• Concentration: 2.2 mg/mL
• Dilution range: ELISA: 1:15,000 - 1:60,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against human FANCA protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with FANCA protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: O15360
• NCBI: 66880553
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-FANCA antibody, FANC-A, FANC A, Fancon
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a main band at approximately 163 kDa in size corresponding to FANCA by western blotting in the appropriate cell lysate or extract or human tissue.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corresponding to FANCA in HeLa whole cell lysates (p/n orb348668). The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1500. The membrane was washed and reacted with a 1:10000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red).

Western blot using Biorbyt's affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection.