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Catalog Number | orb614094 |
---|---|
Category | Antibodies |
Description | FACL4/ACSL4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence of human FACL4/ACSL4 (EPWTPETGLVTDAFKLKRKELR). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 79 kDa |
UniProt ID | O60488 |
Sensitivity | > 5000 cells |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | "Long-chain-fatty-acid--CoA ligase 4; Long-chain a Read more... |
Note | For research use only |
Application notes | "Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U87 cells using anti-ACSL4 antibody(Blue line).The cells were blocked with 10% normal goat serum.And then incubated with rabbit anti-ACSL4 Antibody.
Flow Cytometry analysis of HepG2 cells using anti-ACSL4 antibody(Blue line).The cells were blocked with 10% normal goat serum.And then incubated with rabbit anti-ACSL4
IF analysis of ACSL4 using anti-ACSL4 antibody.
IHC analysis of ACSL4 using anti-ACSL4 antibody.
IHC analysis of ACSL4 using anti-ACSL4 antibody.
WB analysis using anti-ACSL4 antibody.Lane 1:placenta tissue, Lane 2:HepG2 cell, Lane 3:U2OS cell, Lane 4:rat brain tissue, Lane 5:mouse brain tissue
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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