FABP1 Antibody

• Catalog Number: orb1823991
• Category: Antibodies
• Description: Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epithelium (E-FABP, psoriasis-associated FABP, PA-FABP), striated muscle and heart (H-FABP, mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP or FABP1), myelin (M-FABP) and testis (T-FABP). FABP1 (L-FABP) expression is modulated by developmental, hormonal, dietary and pharmacological factors, and is required for cholesterol synthesis and metabolism.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FABP1/4990
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1-127) from the human protein was used as the immunogen for the FABP1 antibody.
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This FABP1 antibody is available for research use only.
• UniProt ID: P07148
• Storage: Aliquot the FABP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney tissue with FABP1 antibody (clone FABP1/4990). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human kidney tissue lysate with FABP1 antibody (clone FABP1/4990). Predicted molecular weight ~14 kDa

SDS-PAGE analysis of purified, BSA-free FABP1 antibody (clone FABP1/4990) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19, 000 full-length human proteins using FABP1 antibody (clone FABP1/4990). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.