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F(ab')2 Sheep IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed
Catalog Number: orb348392
| Catalog Number | orb348392 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Sheep IgG (H&L) antibody (RPE) |
| Species/Host | Donkey |
| Clonality | Polyclonal |
| Tested applications | FC, IF |
| Reactivity | Sheep |
| Isotype | IgG F(ab')2 |
| Immunogen | Anti-Sheep IgG was produced by repeated immunization with sheep IgG whole molecule in donkey. |
| Concentration | 0.5 mg/mL |
| Dilution range | FC: 1:100 - 1:250, IF: 1:100 - 1:250 |
| Form/Appearance | Lyophilized |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG Fc, or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins. |
| Conjugation | RPE |
| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | Donkey F(ab')2 Anti-Sheep IgG Antibody Phycoerythr Read more... |
| Note | For research use only |
| Application notes | Antibody Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Optimal titers for other applications should be determined by the researcher. |
| Expiration Date | 12 months from date of receipt. |
