F(ab')2 Rat IgG (H&L) Antibody Texas Red Conjugated Pre-Adsorbed

• Catalog Number: orb348377
• Category: Antibodies
• Description: F(ab')2 Rat IgG (H&L) antibody (Texas Red)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Rat
• Isotype: IgG F(ab')2
• Immunogen: Rat IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: IF: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
• Conjugation: Texas Red
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey F(ab')2 Anti-Rat IgG Texas Red Conjugated P
• Note: For research use only
• Application notes: F(ab')2 Anti-Rat IgG Texas Red Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Western Blot of Texas Red™ conjugated Donkey F(ab')2 Anti-Rat IgG Pre-Adsorbed secondary antibody. Lane 1: Rat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red™ donkey secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rat IgG. Other band(s): Rat IgG splice variants and isoforms.