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F(ab')2 Rat IgG Fc Antibody Fluorescein Conjugated Pre-Adsorbed
Catalog Number: orb348341
| Catalog Number | orb348341 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Rat IgG F(c) antibody (FITC) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | DOT, FC, FLISA, IF |
| Reactivity | Rat |
| Isotype | IgG F(ab')2 |
| Immunogen | Rat IgG F(c) fragment |
| Concentration | 1.0 mg/mL |
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins. |
| Conjugation | FITC |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | Goat F(ab')2 Anti-Rat IgG F(c) Fluorescein Conjuga Read more... |
| Note | For research use only |
| Application notes | F(ab')2 Anti-Rat IgG F(c) Fluorescein Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot results of Goat F(ab')2 Anti-Rat IgG Antibody Fluorescein Conjugated. Dots are Rat F(c) at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat F(ab')2 Anti-Rat IgG Antibody FITC at 1 µg/ml for 1hr at RT.
