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Catalog Number | orb346799 |
---|---|
Category | Antibodies |
Description | F(ab')2 Rat IgG F(ab')2 antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Rat |
Isotype | IgG F(ab')2 |
Immunogen | Rat IgG F(ab')2 fragment |
Concentration | 10 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit F(ab')2 anti-Rat IgG F(ab')2 peroxidase Con Read more... |
Note | For research use only |
Application notes | F(ab')2 Anti-Rat IgG F(ab')2 Peroxidase Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. |
Expiration Date | 12 months from date of receipt. |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin. | |
Rat |
ELISA, IHC, WB | |
Rat | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Rat | |
Goat | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Rat | |
Goat | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Rat | |
Rabbit | |
Polyclonal | |
HRP |