F(ab')2 Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb348322
• Category: Antibodies
• Description: F(ab')2 Rabbit IgG (H&L) antibody (FITC)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF
• Reactivity: Rabbit
• Isotype: IgG F(ab')2
• Immunogen: Rabbit IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey F(ab')2 Anti-Rabbit IgG Fluorescein Conjuga
• Note: For research use only
• Application notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Vero E6 cells 5 days after inoculation with sample 49 that tested negative by Ag-RDT. Cells were fixed in 4% neutral buffered formalin solution, stained using a rabbit polyclonal anti-SARS-CoV nucleocapsid protein, and examined under a fluorescent immunofluorescence microscope. Scale bar is 50 µm.