F(ab')2 Rabbit IgG F(c) antibody (FITC)

• Catalog Number: orb348304
• Category: Antibodies
• Description: F(ab')2 Rabbit IgG F(c) antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, IF, WB
• Reactivity: Rabbit
• Isotype: IgG F(ab')2
• Immunogen: Rabbit IgG F(c) fragment
• Concentration: 1.0 mg/mL
• Dilution range: FC: 1:500-1:2,500, IF: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab).
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat F(ab')2 Anti-Rabbit IgG F(c) Fluorescein Conj
• Note: For research use only
• Application notes: F(ab')2 Anti-Rabbit IgG F(c) Fluorescein Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Goat F(ab')2 Anti-Rabbit IgG F(c) Antibody Fluorescein Conjugate. Dots are Rabbit IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Goat F(ab')2 Anti-Rabbit IgG F(c) Antibody FITC at 1 ug/ml in orb348637 1hr RT.