F(ab')2 Rabbit IgG F(ab')2 Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb348306
• Category: Antibodies
• Description: F(ab')2 Rabbit IgG F(ab')2 antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF
• Reactivity: Rabbit
• Isotype: IgG F(ab')2
• Immunogen: Rabbit IgG F(ab')2 fragment
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat F(ab')2 Anti-Rabbit IgG F(ab')2 Fluorescein C
• Note: For research use only
• Application notes: F(ab')2 Anti-Rabbit IgG F(ab')2 Fluorescein Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit IgG Fab2 Antibody Fluorescein Conjugated. Antigen: Rabbit IgG. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Rabbit IgG Fab2 Antibody Fluorescein Conjugated at 1:1000 in orb348637 for 60 min at RT. Block: orb348637 for 60 min at RT.