F(ab')2 Mouse IgG (H&L) Antibody Texas Red Conjugated

• Catalog Number: orb348269
• Category: Antibodies
• Description: F(ab')2 Mouse IgG (H&L) antibody (Texas Red)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF
• Reactivity: Mouse
• Isotype: IgG F(ab')2
• Immunogen: Mouse IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Conjugation: Texas Red
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat F(ab')2 Anti-Mouse IgG Antibody Texas Red Con
• Note: For research use only
• Application notes: F(ab')2 Anti-Mouse IgG (H&L) Texas Red Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of F(ab')2 Goat Anti-Mouse IgG Antibody Texas Red conjugated. Mouse IgG (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70, (5) 1.23 ng. Antibody: F(ab')2 Goat Anti-Mouse IgG Antibody Texas Red conjugate at 1 µg/ml for 1hr at RT. Block: Fluorescent Buffer (pn orb348637) for 30 mins at RT.