F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb348249
• Category: Antibodies
• Description: F(ab')2 Mouse IgG (H&L) antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF
• Reactivity: Mouse
• Isotype: IgG F(ab')2
• Immunogen: Mouse IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat F(ab')2 Anti-Mouse IgG Antibody Fluorescein C
• Note: For research use only
• Application notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Characterization of the effects of Akt and c-Myc on mitochondria. FL5.12 parental and FL5.12-AM cells were cultured with DOX or 4-OHT for 36 h. (B) cells were incubated with control IgG or anti-Cox IV subunit 1 and then incubated with fluorescein isothiocyanate-conjugated anti-mouse IgG secondary antibody [orb348249]. The expression level of mitochondrial complex IV subunit 1 was analyzed by flow cytometry. Note that the addition of 4-OHT increased the expression of complex IV subunit 1 in FL5.12-AM but not in the parental cells.