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F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated

F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated

Catalog Number: orb346787

DispatchUsually dispatched within 5-10 working days
$ 530.00
Catalog Numberorb346787
CategoryAntibodies
DescriptionF(ab')2 Mouse IgG (H&L) antibody (Peroxidase)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityMouse
IsotypeIgG F(ab')2
ImmunogenMouse IgG whole molecule
Concentration10.0 mg/mL
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Form/AppearanceLyophilized
PurityThis product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
ConjugationHRP
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Alternative namesRabbit F(ab')2 anti-Mouse IgG Antibody peroxidase
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NoteFor research use only
Application notesSecondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
Expiration Date12 months from date of receipt.