F(ab')2 Mouse IgG Fc Antibody Alkaline Phosphatase Conjugated Antibody Pre-Adsorbed

• Catalog Number: orb348257
• Category: Antibodies
• Description: F(ab')2 Mouse IgG F(c) antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG F(ab')2
• Immunogen: Mouse IgG F(c) fragment
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab) or Bovine, Horse and Human Serum Proteins.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Alternative names: Goat F(ab')2 Anti-Mouse IgG F(c) Antibody Alkaline
• Note: For research use only
• Application notes: Fab')2 Anti-Mouse IgG F(c) Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

ELISA Results of F(ab;)2 Goat Anti-Mouse IgG F(c) Antibody Alkaline Phosphatase Conjugated. Each well was coated in duplicate with 10 µg of Mouse IgG F(c) (p/n orb346280). The working dilution is 40700. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS, NPP ELISA Alkaline Phosphatase Substrate, and TMB substrate (p/n orb348651).