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F(ab')2 Human IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

F(ab')2 Human IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Catalog Number: orb348218

DispatchUsually dispatched within 5-10 working days
$ 430.00
Catalog Numberorb348218
CategoryAntibodies
DescriptionF(ab')2 Human IgG (H&L) antibody (Biotin)
Species/HostDonkey
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityHuman
IsotypeIgG F(ab')2
ImmunogenHuman IgG whole molecule
Concentration1.0 mg/mL
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
Form/AppearanceLyophilized
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat, and Sheep Serum Proteins.
ConjugationBiotin
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.05% (w/v) Sodium Azide
Alternative namesDonkey F(ab')2 Anti-Human IgG biotin Conjugated An
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NoteFor research use only
Application notesF(ab')2 Anti-Human IgG (H&L) Biotin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Expiration Date12 months from date of receipt.