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F(ab')2 Human IgG Fc Antibody Peroxidase Conjugated Pre-Adsorbed
Catalog Number: orb348189
| Catalog Number | orb348189 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Human IgG F(c) antibody (Peroxidase) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Human |
| Isotype | IgG F(ab')2 |
| Immunogen | Human IgG F(c) fragment |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:130,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | F(ab')2 Anti-Human IgG Fc was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins. |
| Conjugation | HRP |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | Goat F(ab')2 Anti-Human IgG F(c) Peroxidase Conjug Read more... |
| Note | For research use only |
| Application notes | F(ab')2 Anti-Human IgG F(c) Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
ELISA results of purified F(ab')2 Goat anti-Human IgG F(c) Antibody Peroxidase Conjugated min x Bv, Hs, Ms, ant Rt serum proteins tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n orb346220). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.
