F(ab')2 Human IgG Fc Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb348183
• Category: Antibodies
• Description: F(ab')2 HUMAN IgG F(c) antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Human
• Isotype: IgG F(ab')2
• Immunogen: Human IgG F(c) fragment
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins.
• Conjugation: FITC
• Biological Activity: 2.8
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat F(ab')2 Anti-Human IgG F(c) Fluorescein Conju
• Note: For research use only
• Application notes: F(ab')2 Anti-Human IgG F(c) Fluorescein Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-F(ab')2 HUMAN IgG F(c) Antibody Fluorescein Conjugated Pre-Adsorbed Lane 1: Human Fc Load: 50 ng per lane Secondary antibody: F(ab')2 HUMAN IgG F(c) Antibody Fluorescein Conjugated Pre-Adsorbed at 1:1000 for 60 min at RT Block: orb348637 for 30 min at RT.