F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

• Catalog Number: orb348127
• Category: Antibodies
• Description: F(ab')2 Goat IgG (H&L) antibody (RPE)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: DOT, FC, IF, WB
• Reactivity: Goat
• Isotype: IgG F(ab')2
• Immunogen: Anti-Goat IgG was produced by repeated immunization with goat IgG whole molecule in donkey.
• Concentration: 1.032 mg/ml
• Dilution range: FC: 1:100 - 1:250, IF: 1:100 - 1:250
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins.
• Conjugation: RPE
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Alternative names: Donkey F(ab')2 Anti-Goat IgG Antibody Phycoerythri
• Note: For research use only
• Application notes: F(ab')2 Anti-Goat IgG Phycoerythrin Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
• Expiration Date: 12 months from date of receipt.

Dot Blot of F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody. Antigen: Goat IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated. Primary antibody: N/A. Secondary antibody: F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody at 1:1000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 60 min at RT.

Dot Blot results of Donkey F(ab')2 Anti-Goat IgG Antibody Phycoerythrin Conjugated. Dots are Goat IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Donkey F(ab')2 Anti-Goat IgG Antibody RPE at 1 µg/ml for 1hr at RT.