F(ab')2 Goat IgG (H&L) Antibody Pre-Adsorbed

• Catalog Number: orb348122
• Category: Antibodies
• Description: F(ab')2 Goat IgG (H&L) antibody
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Goat
• Isotype: IgG F(ab')2
• Immunogen: Goat IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey F(ab')2 Anti-Goat IgG Antibody, Donkey Fab2
• Note: For research use only
• Application notes: F(ab')2 Anti-Goat IgG Antibody has been tested by SDS-PAGE and dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of F(ab')2 Donkey Anti-GOAT IgG [H&L] Antibody Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, Rt Serum Proteins. Goat IgG (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: F(ab')2 Dk-a-Gt IgG Mx8 at 1 µg/ml for 1hr at RT. Secondary Antibody: Goat Anti-Donkey HRP at 1:40000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 30 mins at RT. Exposure: 1 sec.

SDS PAGE Results of F(ab')2 Anti-GOAT IgG (DONKEY) Antibody. Lane 1: Fab2 Dk-a-Gt IgG Mx8 Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Fab2 Dk-a-Gt IgG Mx8 Non-Reduced [1.0 µg]. 4-20% Gel, Coomassie Stained.