F(ab')2 Goat IgG (H&L) Antibody Texas Red Conjugated

• Catalog Number: orb348114
• Category: Antibodies
• Description: F(ab')2 Goat IgG (H&L) antibody (Texas Red)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, IF
• Reactivity: Goat
• Isotype: IgG F(ab')2
• Immunogen: Goat IgG whole molecule
• Concentration: 1 mg/mL
• Dilution range: IF: 1:500-1:2,500
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Conjugation: Texas Red
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit F(ab')2 Anti-Goat IgG Antibody Texas Red Co
• Note: For research use only
• Application notes: F(ab')2 Anti-Goat IgG Texas Red Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot of F(ab)2 Anti-Goat IgG (H&L) Antibody Texas Red Conjugated. Lane 1: 100 ng of Goat IgG. Lanes 2-5: Serial Dilution in 3-fold. Primary Antibody: none. Secondary Antibody: F(ab)2 Rabbit Anti-Goat IgG (H&L) Antibody Texas Red™ Conj'd at 1 mg/ml at RT for 1 hour. Block: orb348637 at RT for 30 minutes.