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Catalog Number | orb348094 |
---|---|
Category | Antibodies |
Description | F(ab')2 Chicken IgG (H&L) antibody (Alkaline Phosphatase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Gallus |
Isotype | IgG F(ab')2 |
Immunogen | Chicken IgG whole molecule |
Concentration | 0.5 mg/mL |
Dilution range | ELISA: 1:4,500, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Chicken IgG and Chicken Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
Conjugation | AP |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit F(ab')2 Anti-Chicken IgG Alkaline Phosphata Read more... |
Note | For research use only |
Application notes | F(ab')2 Chicken IgG (H&L) antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |