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F(ab')2 Bovine IgG (H&L) Antibody Texas Red Conjugated
Catalog Number: orb348080
| Catalog Number | orb348080 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Bovine IgG (H&L) antibody (Texas Red) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, IF |
| Reactivity | Bovine |
| Isotype | IgG F(ab')2 |
| Immunogen | Bovine IgG whole molecule |
| Concentration | 1.0 mg/mL |
| Dilution range | IF: 1:500-1:2,500 |
| Form/Appearance | Lyophilized |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
| Conjugation | Texas Red |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | Rabbit F(ab')2 Anti-Bovine Texas Red Conjugated An Read more... |
| Note | For research use only |
| Application notes | F(ab')2 Anti-Bovine IgG Texas Red Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit anti-Fab2 Bovine IgG Antibody Texas Red Conjugated. Antigen: Bovine IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Fab2 Bovine IgG Antibody Texas Red Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.
