Fab Rabbit IgG (H&L) Antibody Biotin Conjugated

• Catalog Number: orb348486
• Category: Antibodies
• Description: Fab Rabbit IgG (H&L) antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rabbit
• Isotype: IgG Fab
• Immunogen: Rabbit IgG whole molecule
• Concentration: 1.0mg/mL
• Dilution range: ELISA: 1:90,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Conjugation: Biotin
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Fab Anti-Rabbit IgG Biotin Conjugated Antibod
• Note: For research use only
• Application notes: Fab Anti-Rabbit IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Fab Anti-Rabbit IgG (Goat) Antibody Biotin Conjugated. Each well was coated in duplicate with 10 µg of Rabbit IgG (p/n orb2652747). The working dilution is 368000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS, Streptavidin-HRP conjugated (p/n orb348767) and TMB substrate (p/n orb348651).