Fab Rabbit IgG (H&L) Antibody Peroxidase Conjugated

• Catalog Number: orb348485
• Category: Antibodies
• Description: Fab Rabbit IgG (H&L) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rabbit
• Isotype: IgG Fab
• Immunogen: Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:60,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Goat Fab Anti-Rabbit IgG Antibody Peroxidase Conju
• Note: For research use only
• Application notes: Fab Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Western blot of Peroxidase conjugated Goat Fab Anti-Rabbit IgG secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.