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Fab Mouse IgG (H&L) Antibody Texas Red Conjugated

Catalog Number: orb348475

DispatchUsually dispatched within 5-10 working days
$ 380.00
Catalog Numberorb348475
CategoryAntibodies
DescriptionFab Mouse IgG (H&L) antibody (Texas Red)
Species/HostGoat
ClonalityPolyclonal
Tested applicationsDOT, FC, FLISA, IF
ReactivityMouse
IsotypeIgG Fab
ImmunogenMouse IgG whole molecule
Concentration1.0 mg/mL
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Form/AppearanceLyophilized
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
ConjugationTexas Red
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesGoat Fab Anti-Mouse IgG Texas Red Conjugated Antib
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NoteFor research use only
Application notesFab Anti-Mouse IgG (H&L) Texas Red Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Expiration Date12 months from date of receipt.
Fab Mouse IgG (H&L) Antibody Texas Red Conjugated

Dot Blot of Fab Anti-MOUSE IgG (GOAT) Texas Red™ Conjugated Antibody. Lane 1: 100 ng. Lane 2: 33.3 ng. Lane 3: 11.1 ng. Lane 4: 3.7 ng. Lane 5: 1.23 ng. Secondary Antibody: Goat anti-Mouse IgG Texas Red. Blocking Buffer: BlockOut orb348644 for 30 min at RT.