F10 Antibody

• Catalog Number: orb1259913
• Category: Antibodies
• Description: F10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 235-488 of human F10 (NP_000495.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 75kDa
• Target: F10
• UniProt ID: P00742
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: F10, FX, FXA
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using F10 antibody (orb1259913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of A549 cells using F10 antibody (orb1259913). Blue: DAPI for nuclear staining.