You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1173509 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-E3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-E3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-E3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Tested applications | ELISA |
Reactivity | All |
Range | 0.63-40 ng/mL |
Sensitivity | 0.218 ng/mL |
Protein Length | 2h |
Alternative names | F-E3; Free Estriol Read more... |
Note | For research use only |
Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Filter by Rating