Ezrin Antibody / EZR

• Catalog Number: orb606931
• Category: Antibodies
• Description: Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. Overexpression of Ezrin predicts the poor prognosis of gastric adenocarcinoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-Ezrin-1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full length human protein was used as the immunogen for the Ezrin antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Ezrin antibody is available for research use only.
• UniProt ID: P15311
• Storage: Store the Ezrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Ezrin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma with Ezrin antibody. Required HIER: boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to staining.

IHC testing of FFPE human placenta with Ezrin antibody. Required HIER: boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to staining.

Western blot testing of human SK-BR-3, HeLa, HEK293 and MCF-7 cell lysate with Ezrin antibody. Expected molecular weight: 70-80 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Ezrin antibody (clone CPTC-Ezrin-1). These results demonstrate the foremost specificity of the CPTC-Ezrin-1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.