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Catalog Number | orb1672047 |
---|---|
Category | Antibodies |
Description | EZR Antibody |
Clonality | Recombinant |
Clone Number | SAIC-37C-23 |
Tested applications | WB |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | The KLH-conjugated peptide "SGYLSSER" derived from Ezrin, a protein likely involved in connections of major cytoskeletal structures to the plasma membrane. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | EZR |
UniProt ID | P15311 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Ezrin, VIL2 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry using the Anti-EZR antibody SAIC-37C-23. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-37C-23 (orb1672047 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of HeLa cells with anti-EZR (SAIC-37C-23). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric r version of SAIC-37C-23 (orb1672047) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672047- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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