EZH2 Antibody

• Catalog Number: orb1822916
• Category: Antibodies
• Description: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EZH2/6988
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 300-550) from the human protein was used as the immunogen for the Enhancer of zeste homolog 2 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This Enhancer of zeste homolog 2 antibody is available for research use only.
• UniProt ID: Q15910
• Storage: Aliquot the EZH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human bladder carcinoma with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Lynch syndrome colon carcinoma tissue with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder carcinoma with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Jurkat cell lysate with Enhancer of zeste homolog 2 antibody (clone EZH2/6988). Expected molecular weight: 85-95 kDa.