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Catalog Number | orb1821419 |
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Category | Tools |
Description | Extreme Thermolabile UNG (UDG) is ideal for use in one-step RT-PCR systems to remove contaminations with uracil-containing DNA before onset of the reverse transcription. The enzyme is fully active at temperatures between 15 to 25°C and inactivated at 50°C. Extreme Thermolabile UNG is a recombinant uracil-DNA glycosylase from marine bacterium BMTU 3346. UNG catalyzes the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. An amount of 1 unit UNG completely digests 104to 106copies of U-containing DNA fragments in 2 min at 50°C. |
Concentration | 1 unit/µl |
Form/Appearance | liquid |
Storage | store at -20 °C. avoid freeze/thaw cycles |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |