Estrogen Receptor α (ready to use) Mouse Monoclonal Antibody

• Catalog Number: orb544741
• Category: Antibodies
• Description: Estrogen Receptor α (ready to use) Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7G2
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: human Estrogen Receptor α
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 66 kDa
• Target: ESR1
• UniProt ID: P03372
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 1% rAlbumin and 0.02% Proclin300.
• Alternative names: Estradiol receptor; Estradiol Receptor-alpha; Estr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Estrogen Receptor α) Monoclonal Antibody, Unconjugated (orb544741) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Estrogen Receptor α) Monoclonal Antibody, Unconjugated (orb544741) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human endometrial), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Estrogen Receptor α) Monoclonal Antibody, Unconjugated (orb544741) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Estrogen Receptor α) Monoclonal Antibody, Unconjugated (orb544741) overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.