Estrogen Receptor beta Antibody [ERb455]

• Catalog Number: orb1252769
• Category: Antibodies
• Description: Estrogen Receptor beta Antibody [ERb455]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ERb455
• Tested applications: FC, IF, IHC, WB
• Reactivity: Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep
• Isotype: IgG2a
• Immunogen: The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ESR2
• UniProt ID: Q92731
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Estrogen receptor beta, ER-beta, Nuclear receptor
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of breast cancer stained with Estrogen Receptor beta antibody (ERb455).

IHC testing of ovarian cancer stained with Estrogen Receptor beta antibody (ERb455).

FACS testing of MCF-7 cells: Black = cells alone; Green = isotype control; Red = Estrogen Receptor beta antibody PE conjugate.

FACS testing of BT474 cells: Black = cells alone; Gray = isotype control; Green = Estrogen Receptor beta antibody AF488 conjugate.

IHC testing of gastric carcinoma stained with Estrogen Receptor beta antibody (ERb455).

IHC testing of bladder carcinoma stained with Estrogen Receptor beta antibody (ERb455).

SDS-PAGE Analysis of Purified, BSA-Free Estrogen Receptor beta Antibody (clone ERb455). Confirmation of Integrity and Purity of the Antibody.