Estrogen Receptor alpha Rabbit Polyclonal Antibody

• Catalog Number: orb10602
• Category: Antibodies
• Description: Estrogen Receptor alpha Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse ER-alpha (501-600/600aa)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2g/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 67 kDa
• Target: ESR1
• UniProt ID: P19785
• RRID: AB_10928255
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Estradiol receptor; Estrogen receptor alpha; Estra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wu, Yingxi et al. Downregulation of G‑protein‑coupled receptor 30 in the hippocampus attenuates the neuroprotection of estrogen in the critical period hypothesis Mol Med Rep, 17, 5716-5725 (2018)

PubMed: 29484405

Blank control (blue line): MCF7 (blue). Primary Antibody (green line): Rabbit Anti-Estrogen Receptor alpha antibody (orb10602), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 80% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Estrogen Receptor alpha) Polyclonal Antibody, Unconjugated (orb10602) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Rat) lysate at 30 ug, Primary: Anti-ER-alpha/beta (orb10602) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution (DAB staining), Predicted band size: 67kD, Observed band size: 67kD.