Estrogen Receptor alpha Antibody

• Catalog Number: orb639507
• Category: Antibodies
• Description: This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ESR1/2299R
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Estrogen Receptor antibody is available for research use only.
• UniProt ID: P03372
• Storage: Store the recombinant Estrogen Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant Estrogen Receptor antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human breast cancer with Estrogen Receptor antibody (clone ESR1/2299R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human MCF7 cell lysate with recombinant Estrogen Receptor antibody (clone ESR1/2299R). Predicted molecular weight ~67 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Estrogen Receptor antibody (clone ESR1/2299R) as confirmation of integrity and purity.

Flow cytometry staining of PFA-fixed human MCF7 cells with recombinant Estrogen Receptor antibody; Red=isotype control, Blue=recombinant Estrogen Receptor antibody.