Estrogen Receptor alpha Antibody

• Catalog Number: orb607139
• Category: Antibodies
• Description: This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ESR1/3557
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human ESR1 protein fragment within amino acids 129-312 was used as the immunogen for the Estrogen Receptor alpha antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Estrogen Receptor alpha antibody is available for research use only.
• UniProt ID: P03372
• Storage: Store the Estrogen Receptor alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of human MCF-7 cells with Estrogen Receptor alpha antibody (green, clone ESR1/3557) and Phalloidin (red).

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of fixed and permeabilized human MCF-7 cells with Estrogen Receptor alpha antibody (clone ESR1/3557); Red=isotype control, Blue= Estrogen Receptor alpha antibody.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Estrogen Receptor alpha antibody (clone ESR1/3557). These results demonstrate the foremost specificity of the ESR1/3557 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/3557) as confirmation of integrity and purity.