Estrogen Receptor alpha Antibody

• Catalog Number: orb606409
• Category: Antibodies
• Description: This mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rESR1/1935
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor alpha antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Estrogen Receptor alpha antibody is available for research use only.
• UniProt ID: P03372
• Storage: Store the recombinant Estrogen Receptor alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant Estrogen Receptor alpha antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of MCF7 cell lysate with recombinant Estrogen Receptor alpha antibody. Predicted molecular weight ~66 kDa.

IHC testing of FFPE human breast cancer with Estrogen Receptor alpha antibody (clone rESR1/1935). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Estrogen Receptor alpha antibody (clone rESR1/1935) as confirmation of integrity and purity.